Journal of Bioscience and Bioengineering 2013-02-01

Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter.

Eiji Nagamori, Hideaki Fujita, Kazunori Shimizu, Kenro Tokuhiro, Nobuhiro Ishida, Haruo Takahashi

Index: J. Biosci. Bioeng. 115(2) , 193-5, (2013)

Full Text: HTML

Abstract

A simple fed-batch system for cultivating genetically engineered yeast generating lactate under the regulation of the PDC1 promoter was established. Traditional strategies that avoid occurrence of Crabtree effect, such as respiratory quotient (RQ) control or ethanol control, are not applicable to the strain because of reduced generation of ethanol and CO(2) by-products. In this system, the feed rate increased when the pH was >5.0, and decreased when the pH was <5.0. Using this system, cell yields on sucrose increased by approximately 30% compared to that with the conventional RQ control method, due to the early detection of occurrence of Crabtree effect by pH decrease.Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Related Compounds

  • Pyruvate decarboxy...

Related Articles:

Pyruvate decarboxylase activity is regulated by the Ser/Thr protein phosphatase Sit4p in the yeast Saccharomyces cerevisiae.

2013-09-01

[FEMS Yeast Res. 13(6) , 518-28, (2013)]

Computational, structural, and kinetic evidence that Vibrio vulnificus FrsA is not a cofactor-independent pyruvate decarboxylase.

2013-03-19

[Biochemistry 52(11) , 1842-4, (2013)]

Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae.

2012-05-31

[J. Biotechnol. 159(1-2) , 32-7, (2012)]

Substrate specificity of thiamine pyrophosphate-dependent 2-oxo-acid decarboxylases in Saccharomyces cerevisiae.

2012-11-01

[Appl. Environ. Microbiol. 78(21) , 7538-48, (2012)]

Chlamydomonas reinhardtii chloroplasts contain a homodimeric pyruvate:ferredoxin oxidoreductase that functions with FDX1.

2013-01-01

[Plant Physiol. 161(1) , 57-71, (2013)]

More Articles...