Sulfation of various alcoholic groups by an arylsulfate sulfotransferase from desulfitobacterium hafniense and synthesis of estradiol sulfate

MA van der Horst, JFT van Lieshout…

Index: Van Der Horst, Michael A.; Van Lieshout, Johan F. T.; Bury, Aleksandra; Hartog, Aloysius F.; Wever, Ron Advanced Synthesis and Catalysis, 2012 , vol. 354, # 18 p. 3501 - 3508

Full Text: HTML

Citation Number: 11

Abstract

Abstract Bacterial arylsulfate sulfotransferases (AST) are enzymes that catalyse the transfer of a sulfate group from p-nitrophenyl sulfate (p-NPS) to a phenolic acceptor molecule. By screening of the NCBI protein database a gene coding for an AST was found in Desulfitobacterium hafniense. After expression the enzyme was purified and characterised. This AST efficiently sulfates various acceptor molecules (estrone, estradiol, enkephalin ...