Purification and characterization of beta-mannosidases from white rot fungus Phlebia radiata.

Monika Prendecka, Agnieszka Buczyńska, Jerzy Rogalski

Index: Pol. J. Microbiol. 56 , 139-147, (2007)

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Abstract

A beta-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50 degrees C. Highly purified isoforms of beta-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Sepharose and chromatofocusing on Polybuffer Exchanger PBE-94.