Biochemical and Biophysical Research Communications 1998-03-27

Induction of CYP102 (cytochrome P450BM-3) in Bacillus megaterium by 17 beta-estradiol and 4-sec-butylphenol.

N E Hopkins, N English, V Hughes, C W Rowley, C R Wolf, W L Alworth

Index: Biochem. Biophys. Res. Commun. 244(3) , 868-72, (1998)

Full Text: HTML

Abstract

CYP102 (Cytochrome P450BM-3) is induced in Bacillus megaterium by barbiturates, peroxisome proliferators, and nonsteroidal anti-inflammatory drugs. We now describe the induction of CYP102 in B. megaterium by 17 beta-estradiol and by 4-sec-butylphenol. These estrogens interact with the repressor protein Bm3R1, causing it to dissociate with the operator of the CYP102 gene and allowing transcription to occur. We have developed a stable transfection of a construct into B. megaterium of a truncated CYP102 gene coupled with the luciferase gene in a promoterless plasmid and have used this construct to test the induction of CYP102 by these estrogens. Estradiol demonstrated a dose-dependent induction of CYP102 which saturated at a 2-fold increase at 150 microM 4 hr post-addition. 4-sec-Butylphenol produced a dose-dependent and time-dependent induction up to 300 microM and 6 hr post-induction.

Related Compounds

  • 4-(2-Butyl)phenol
  • 2-sec-Butylphenol

Related Articles:

Convenient QSAR model for predicting the complexation of structurally diverse compounds with β-cyclodextrins

2009-01-01

[Bioorg. Med. Chem. 17 , 896-904, (2009)]

Impact of induced fit on ligand binding to the androgen receptor: a multidimensional QSAR study to predict endocrine-disrupting effects of environmental chemicals.

2005-09-08

[J. Med. Chem. 48 , 5666-74, (2005)]

Biotransformation of 4-sec-butylphenol by Gram-positive bacteria of the genera Mycobacterium and Nocardia including modifications on the alkyl chain and the hydroxyl group.

2013-09-01

[Appl. Microbiol. Biotechnol. 97(18) , 8329-39, (2013)]

More Articles...