BioTechniques 2000-05-01

Detection of Glu-Glu-tagged proteins in mammalian cell culture media by dot immunoblotting.

J L Ellsworth, N Hamacher, N Bagwell, J W West

Index: Biotechniques 28(5) , 934-6, 937, (2000)

Full Text: HTML

Abstract

A dot immunoblotting technique has been developed to estimate the relative expression levels of tagged recombinant human proteins in mammalian cell culture media. Variations in sample denaturation, blocking agents and membrane composition and treatment were used to optimize the signal-to-noise ratio of the defined procedure. The method is rapid, with sensitivity extending to the low nanomolar range for a number of recombinant proteins. This technique should have general utility for antibody-based measurements of other tagged and non-tagged proteins in cell culture media or in biological fluids.

Related Compounds

  • H-Glu-Glu-OH

Related Articles:

Umami-bitter interactions: the suppression of bitterness by umami peptides via human bitter taste receptor.

2015-01-09

[Biochem. Biophys. Res. Commun. 456(2) , 586-90, (2015)]

Combined PKC and MEK inhibition in uveal melanoma with GNAQ and GNA11 mutations.

2014-09-25

[Oncogene 33(39) , 4724-34, (2014)]

Complex expression of the UL136 gene of human cytomegalovirus results in multiple protein isoforms with unique roles in replication.

2014-12-01

[J. Virol. 88(24) , 14412-25, (2014)]

Studies on selection blockers. 5. Design, synthesis, and biological profile of sialyl Lewis x mimetics based on modified serine-glutamic acid dipeptides.

1997-10-24

[J. Med. Chem. 40 , 3534-3541, (1997)]

Acidic peptides enhanced genistein-dependent inhibition of human platelet aggregation: potential protective effect of digestible peptides plus genistein against atherosclerosis.

2008-08-01

[Nutr. Res. 28(8) , 523-31, (2008)]

More Articles...