Applied Microbiology and Biotechnology 2010-03-01

The mechanisms of citrate on regulating the distribution of carbon flux in the biosynthesis of uridine 5'-monophosphate by Saccharomyces cerevisiae.

Yong Chen, Shuya Li, Jian Xiong, Zhenjiang Li, Jianxin Bai, Lei Zhang, Qi Ye, Pingkai Ouyang, Hanjie Ying

Index: Appl. Microbiol. Biotechnol. 86 , 75-81, (2010)

Full Text: HTML

Abstract

A whole cell biocatalytic process for uridine 5'-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. The concentration of UMP was increased by 23% when 1 g l(-1) sodium citrate was fed into the broth. Effects of citrate addition on UMP production were investigated. Glucose-6-phosphate pool was elevated by onefold, while FBP and pyruvate were decreased by 42% and 40%, respectively. Organic acid pools such as acetate and succinate were averagely decreased by 30% and 49%. The results demonstrated that manipulation of citrate levels could be used as a novel tool to regulate the metabolic fluxes distribution among glycolysis, pentose phosphate pathway, and TCA cycle.

Related Compounds

  • Orotic acid
  • Orotic acid potass...

Related Articles:

Solubilization of gliadins for use as a source of nitrogen in the selection of bacteria with gliadinase activity.

2015-02-01

[Food Chem. 168 , 439-44, (2014)]

Cheminformatics analysis of assertions mined from literature that describe drug-induced liver injury in different species.

2010-01-01

[Chem. Res. Toxicol. 23 , 171-83, (2010)]

QSPR modeling of octanol/water partition coefficient for vitamins by optimal descriptors calculated with SMILES.

2008-04-01

[Eur. J. Med. Chem. 43 , 714-40, (2008)]

Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography.

2015-04-03

[J. Chromatogr. A. 1388 , 110-8, (2015)]

High-throughput tandem mass spectrometry multiplex analysis for newborn urinary screening of creatine synthesis and transport disorders, Triple H syndrome and OTC deficiency.

2014-09-25

[Clin. Chim. Acta 436 , 249-55, (2014)]

More Articles...