Enantioselective drug monitoring of (R)- and (S)- propranolol in human plasma via derivatization with optically active (R,R)-O,O-diacetyl tartaric acid anhydride.

W Lindner, M Rath, K Stoschitzky, G Uray

Index: J. Chromatogr. A. 487 , 375, (1989)

Full Text: HTML

Abstract

A sensitive high-performance liquid chromatographic method was developed for the stereoselective assay of (R)- and (S)-propranolol in human plasma. The method involves diethyl ether extraction of the drugs and a racemic internal standard, N-tert.-butylpropranolol, followed by derivatization of the compounds with the chiral reagent (R,R)-O,O-diacetyl tartaric acid anhydride. The resulting diastereomeric derivatives were separated isocratically on a reversed-phase column. Quantitation was achieved by the peak-height ratio method with reference to the internal standard. The assay was accurate and reproducible in the concentration range 1-100 ng of (R)- and (S)-propranolol per ml plasma, using fluorescence detection at lambda ex 290 nm and lambda em 335 nm. The applicability of this method was demonstrated for the determination of concentration-time profiles of propranolol enantiomers in the course of comparative pharmacokinetic studies.

Related Compounds

  • (+)-DIACETYL-L...

Related Articles:

Analytical and semi-preparative high-performance liquid chromatographic separation and assay of hydroxychloroquine enantiomers.

[J. Chromatogr. A. 581(1) , 83-92, (1992)]

Measurement of urinary D- and L-2-hydroxyglutarate enantiomers by stable-isotope-dilution liquid chromatography-tandem mass spectrometry after derivatization with diacetyl-L-tartaric anhydride.

2004-08-01

[Clin. Chem. 50(8) , 1391-5, (2004)]

Improved separation of diastereomeric derivatives of enantiomers by a physical network of linear polyvinylpyrrolidone applied as pseudophase in capillary zone electrophoresis.

1994-06-01

[Electrophoresis 15(6) , 769-73, (1994)]

2-hydroxyglutarate production, but not dominant negative function, is conferred by glioma-derived NADP-dependent isocitrate dehydrogenase mutations.

2011-01-01

[PLoS ONE 6 , e16812, (2011)]

A liquid chromatographic assay for the stereospecific quantitative analysis of halofantrine in human plasma.

1995-06-01

[J. Pharm. Biomed. Anal. 13(7) , 911-8, (1995)]

More Articles...