Journal of AOAC International 1996-01-01

Liquid chromatographic determination of tocopherols and tocotrienols in margarine, infant foods, and vegetables.

E J Konings, H H Roomans, P R Beljaars

Index: J. AOAC Int. 79(4) , 902-6, (1996)

Full Text: HTML

Abstract

A liquid chromatographic (LC) method was developed for determination of tocopherols and tocotrienols in foods. Tocopherols and tocotrienols were released after saponification of test portions for 40 min at 80 degrees C, followed by extraction with hexane-ethyl acetate. After evaporation of the organic solvents, the extract was injected in the LC system. Separation of individual tocopherols and tocotrienols was satisfactory. Some interferences were encountered from tocomoneols, tocodienols, 2-tert-butyl-4-hydroxyanisole (BHA), 2,6-di-tert-butyl-4-methylphenol, (BHT), and plastochromanol-8. The response of the LC system was linear over a range of 0-10 micrograms/mL for tocopherols and tocotrienols. The detection limit for these compounds was 0.1 micrograms/mL. Repeatabilty relative standard deviation values for tocopherols and tocotrienols in margarine, infant formula, and broccoli (concentration range, 0.11-22 mg/100 g) varied from 1.3 to 6.4%. Recoveries ranged from 91 to 105%.

Related Compounds

  • e-Tokoferol

Related Articles:

Novel HPLC analysis of tocopherols, tocotrienols, and cholesterol in tissue.

1999-12-01

[Free Radic. Biol. Med. 27(11-12) , 1137-40, (1999)]

The tocopherol, tocotrienol, and vitamin E content of the average Finnish diet.

1991-01-01

[Int. J. Vitam. Nutr. Res. 61(1) , 27-32, (1991)]

Oral toxicity of a tocotrienol preparation in rats.

2001-08-01

[Food Chem. Toxicol. 39(8) , 799-805, (2001)]

Selective excretion of yolk-derived tocotrienols into the bile of the chick embryo.

1998-12-01

[Comp. Biochem. Physiol. B Biochem. Mol. Biol. 121(4) , 393-6, (1998)]

Induction of apoptosis in human breast cancer cells by tocopherols and tocotrienols.

1999-01-01

[Nutr. Cancer 33(1) , 26-32, (1999)]

More Articles...