[Ultrastructural and biochemical changes in Mycobacterium rubrum and Streptomyces bacillaris cells exposed to the herbicide semeron].

M N Poglazova, E E Emnova, V A Kodrian, N A Kostrikina, T P Svetlichnaia

Index: Mikrobiologiia 55(5) , 825-30, (1986)

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Abstract

The work was aimed at studying how the herbicide semerone affected the ultrastructure of two soil microorganisms, Mycobacterium rubrum and Streptomyces bacillaris. Depending on its concentration, the herbicide inhibited growth processes so that biomass yield decreased, cell division was interfered with, and giant and misshapen cells appeared. The herbicide taken at a concentration of 50-100 mg/ml increased the amount of membrane structures of the respiration type in some cells. This compound at a concentration of 400-500 mg/ml changed the nucleoid structure in certain cells. The decrease number of ribosomes and their peculiar distribution in the cell cytoplasm are most typical responses of the cells to the herbicide action. These responses were found in all cells at any of the tested herbicide concentrations. The results of cytological experiments are supported by statistically reliable data on the effect of the herbicide on RNA and protein synthesis. RNA synthesis is inhibited at a semerone concentration as low as 1 mg/ml, which is a very sensitive indicator of its presence in the medium.