Journal of Analytical Toxicology 1997-01-01

Detection of 2-amino-5-chloropyridine in urine as a parameter of zopiclone (Imovane) intake using HPLC with diode array detection.

E Mannaert, J Tytgat, P Daenens

Index: J. Anal. Toxicol. 21(3) , 208-12, (1997)

Full Text: HTML

Abstract

A qualitative screening technique was developed for the detection of 2-amino-5-chloropyridine, a newly identified decomposition product of zopiclone and metabolites after alkaline hydrolysis of urine samples. The method was elaborated using a standard operation procedure (Merck Tox Screening System), combining a solid-phase extraction with reversed-phase high-performance liquid chromatography and diode array detection. The limit of detection, expressed as zopiclone concentrations in spiked urine, was found to be 0.5 microg/mL. Field urine samples from a volunteer were positive for 2-amino-5-chloropyridine until 16 h after ingestion of one therapeutic dose of Imovane (7.5 mg zopiclone).

Related Compounds

  • 2-Amino-5-chloropy...

Related Articles:

Simultaneous determination of zopiclone and its degradation product and main impurity (2-amino-5-chloropyridine) by micellar liquid chromatography with time-programmed fluorescence detection: preliminary investigation for biological monitoring.

2012-10-15

[J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 907 , 49-55, (2012)]

Capillary electrophoretic enantioselective determination of zopiclone and its impurities.

2012-06-01

[Electrophoresis 33(11) , 1606-12, (2012)]

Degradation of zopiclone during storage of spiked and authentic whole blood and matching dried blood spots.

2013-01-01

[Int. J. Legal Med. 127(1) , 69-76, (2013)]

Stability tests of zopiclone in whole blood.

2010-07-15

[Forensic Sci. Int. 200(1-3) , 130-5, (2010)]

Zopiclone misuse: an update from Dublin.

2007-01-01

[Drug Alcohol Rev. 26(1) , 83-5, (2007)]

More Articles...