Biochemistry international 1990-09-01

The mechanism of initiation of chondroitin sulphate synthesis by beta-D-galactosides.

M Bradbeer, J A Robinson, H C Robinson

Index: Biochem. Int. 21(6) , 1161-8, (1990)

Full Text: HTML

Abstract

Embryonic chicken cartilage was incubated in vitro with phenyl-beta-[6-3H]galactoside and cycloheximide. Free chondroitin sulphate chains initiated by galactoside were isolated and degraded to yield 3-O-beta-D-glucuronosyl D-galactose (GlcA-Gal) derived from the sequence, GlcA-Gal-Gal-Xyl-Ser, which links the polysaccharide to protein. This enabled the distribution of 3H into specific galactose residues within the linkage oligosaccharide to be determined. Most of the 3H label (65%) was released as free galactose although 35% was recovered as GlcA-Gal. Similar experiments performed with unlabelled phenyl-beta-galactoside and [14C]galactose yielded 14C-labelled GlcA-Gal as a major product. We conclude that beta-galactosides initiate chondroitin sulphate synthesis primarily by serving as substrates for galactosyl transferase II.

Related Compounds

  • Phenylgalactoside

Related Articles:

Fibroblast growth factor 21 protects mouse brain against D-galactose induced aging via suppression of oxidative stress response and advanced glycation end products formation.

2015-06-01

[Pharmacol. Biochem. Behav. 133 , 122-31, (2015)]

Structure-based drug design and optimization of mannoside bacterial FimH antagonists.

2010-06-24

[J. Med. Chem. 53 , 4779-92, (2010)]

Transdifferentiation of mouse adipose-derived stromal cells into acinar cells of the submandibular gland using a co-culture system.

2015-05-15

[Exp. Cell Res. 334 , 160-72, (2015)]

Drosophila KASH-domain protein Klarsicht regulates microtubule stability and integrin receptor localization during collective cell migration.

2015-11-01

[Dev. Biol. 407 , 103-14, (2015)]

COX-2 suppresses tissue factor expression via endocannabinoid-directed PPARdelta activation.

2007-09-03

[J. Exp. Med. 204 , 2053-61, (2007)]

More Articles...