FEBS Letters 2013-11-01

Phylogenetic analysis and substrate specificity of GH2 β-mannosidases from Aspergillus species.

Sumitha K Reddy, Anna Rosengren, Sylvia Klaubauf, Tejas Kulkarni, Eva Nordberg Karlsson, Ronald P de Vries, Henrik Stålbrand

Index: FEBS Lett. 587(21) , 3444-9, (2013)

Full Text: HTML

Abstract

Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised β-mannosidases from other organisms, clusters putative Aspergillus β-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched β-mannosides like mannobiose. A 3D-model of MndB provides further insight. Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Related Compounds

  • β-Mannosidase

Related Articles:

Expression of lignocellulolytic enzymes in Pichia pastoris.

2012-01-01

[Microb. Cell Fact. 11 , 61, (2012)]

Expression and characterization of a Bifidobacterium adolescentis beta-mannanase carrying mannan-binding and cell association motifs.

2013-01-01

[Appl. Environ. Microbiol. 79(1) , 133-40, (2013)]

Lipidemic responses of male broiler chickens to enzyme-supplemented wheat-soybean meal-based diets with various levels of metabolizable energy.

2013-11-01

[Pak. J. Biol. Sci. 16(21) , 1295-302, (2013)]

Cloning, expression, and characterization of β-mannanase from Bacillus subtilis MAFIC-S11 in Pichia pastoris.

2013-04-01

[Appl. Biochem. Biotechnol. 169(8) , 2326-40, (2013)]

Novel low-temperature-active, salt-tolerant and proteases-resistant endo-1,4-β-mannanase from a new Sphingomonas strain.

2012-05-01

[J. Biosci. Bioeng. 113(5) , 568-74, (2012)]

More Articles...