Extremophiles 2006-12-01

Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus.

Stefan Söllner, Silvia Berkner, Georg Lipps

Index: Extremophiles 10(6) , 629-34, (2006)

Full Text: HTML

Abstract

A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography. The enzyme cuts DNA at the recognition site GCwGC as could be shown by restriction analysis of plasmids and short synthetic duplex DNA. The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C.

Related Compounds

  • AVILAMYCIN

Related Articles:

Purification of host cell enzymes involved in adeno-associated virus DNA replication.

2007-06-01

[J. Virol. 81(11) , 5777-87, (2007)]

Topoisomerase V from Methanopyrus kandleri.

2001-01-01

[Meth. Enzymol. 334 , 179-92, (2001)]

Analysis of DNA binding by the adenovirus type 5 E1A oncoprotein.

2002-03-01

[J. Gen. Virol. 83(Pt 3) , 517-24, (2002)]

Porcine islet cells microencapsulated in sodium cellulose sulfate.

2005-01-01

[Transplant. Proc. 37(1) , 248-52, (2005)]

Assay of intrinsic transcript termination by E. coli RNA polymerase on single-stranded and double-stranded DNA templates.

2003-01-01

[Meth. Enzymol. 371 , 339-51, (2003)]

More Articles...