Opposing influences by subsite − 1 and subsite + 1 residues on relative xylopyranosidase/arabinofuranosidase activities of bifunctional β-D-xylosidase/α-L-arabinofuranosidase

Douglas B. Jordan, Jay D. Braker, Douglas B. Jordan, Jay D. Braker

Index: Biochim. Biophys. Acta 1814(12) , 1648-57, (2011)

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Abstract

Conformational inversion occurs 7–8 kcal/mol more readily in furanoses than pyranoses. This difference is exploited here to probe for active-site residues involved in distorting pyranosyl substrate toward reactivity. Spontaneous glycoside hydrolysis rates are ordered 4-nitrophenyl-α- l-arabinofuranoside (4NPA) > 4-nitrophenyl-β- d-xylopyranoside (4NPX) > xylobiose ( X2). The bifunctional β- d-xylosidase/α- l-arabinofuranosidase exhibits the opposite order of reactivity, illustrating that the enzyme is well equipped in using pyranosyl groups of natural substrate X2 in facilitating glycoside hydrolysis. Probing the roles of all 17 active-site residues by single-site mutation to alanine and by changing both moieties of substrate demonstrates that the mutations of subsite − 1 residues decrease the ratio k cat 4NPX/4NPA, suggesting that the native residues support pyranosyl substrate distortion, whereas the mutations of subsite + 1 and the subsite − 1/+1 interface residues increase the ratio k cat 4NPX/4NPA, suggesting that the native residues support other factors, such as C1 migration and protonation of the leaving group. Alanine mutations of subsite − 1 residues raise k cat X2/4NPX and alanine mutations of subsite + 1 and interface residues lower k cat X2/4NPX. We propose that pyranosyl substrate distortion is supported entirely by native residues of subsite − 1. Other factors leading to the transition state are supported entirely by native residues of subsite + 1 and interface residues.