Biochemical and Biophysical Research Communications 2011-07-15

TRPV1 agonist piperine but not olvanil enhances glutamatergic spontaneous excitatory transmission in rat spinal substantia gelatinosa neurons.

Liu Yang, Tsugumi Fujita, Chang-Yu Jiang, Lian-Hua Piao, Hai-Yuan Yue, Kotaro Mizuta, Eiichi Kumamoto

Index: Biochem. Biophys. Res. Commun. 410(4) , 841-5, (2011)

Full Text: HTML

Abstract

We examined the effects of TRPV1 agonists olvanil and piperine on glutamatergic spontaneous excitatory transmission in the substantia gelatinosa (SG) neurons of adult rat spinal cord slices with the whole-cell patch-clamp technique. Bath-applied olvanil did not affect the frequency and amplitude of spontaneous excitatory postsynaptic current (sEPSC), and unchanged holding currents at -70 mV. On the other hand, superfusing piperine reversibly and concentration-dependently increased sEPSC frequency (half-maximal effective concentration: 52.3 μM) with a minimal increase in its amplitude. This sEPSC frequency increase was almost repetitive at an interval of more than 20 min. Piperine at a high concentration produced an inward current in some neurons. The facilitatory effect of piperine was blocked by TRPV1 antagonist capsazepine. It is concluded that piperine but not olvanil activates TRPV1 channels in the central terminals of primary-afferent neurons, resulting in an increase in the spontaneous release of l-glutamate onto SG neurons.Crown Copyright © 2011. Published by Elsevier Inc. All rights reserved.

Related Compounds

  • Olvanil

Related Articles:

Inhibition by capsaicin and its related vanilloids of compound action potentials in frog sciatic nerves.

2013-03-14

[Life Sci. 92(6-7) , 368-78, (2013)]

Identification of drug modulators targeting gene-dosage disease CMT1A.

2012-07-20

[ACS Chem. Biol. 7(7) , 1205-13, (2012)]

Activation of recombinant human TRPV1 receptors expressed in SH-SY5Y human neuroblastoma cells increases [Ca(2+)](i), initiates neurotransmitter release and promotes delayed cell death.

2007-08-01

[J. Neurochem. 102(3) , 801-11, (2007)]

Olvanil, a non-pungent vanilloid enhances the gastrointestinal toxicity of cisplatin in the ferret.

2010-02-15

[Toxicol. Lett. 192(3) , 402-7, (2010)]

Signaling mechanisms of down-regulation of voltage-activated Ca2+ channels by transient receptor potential vanilloid type 1 stimulation with olvanil in primary sensory neurons.

2006-08-11

[Neuroscience 141(1) , 407-19, (2006)]

More Articles...