Journal of Steroid Biochemistry 1985-02-01

Specific antisera for the radioimmunoassay of estradiol-3-sulfate.

T Tanaka, N Suguro, Y Shiratori, A Kubodera

Index: J. Steroid Biochem. 22(2) , 285-8, (1985)

Full Text: HTML

Abstract

Antisera were prepared against two types of estradiol-3-sulfate-bovine serum albumin (BSA) conjugates. The haptens were coupled to BSA through the C-6 position in the steroid molecule by the glutaraldehyde (A) or the carbodiimide method (B). In comparison the antiserum produced by method A had a high affinity for estradiol-3-sulfate (Ka = 5.64 X 10(8) M-1); that produced by method B had an even higher affinity (Ka = 2.62 X 10(9) M-1). Furthermore the latter had no significant cross-reaction with other estrogen sulfates (less than 3.83%), and no cross-reaction with other steroids (less than 0.03%). The former revealed a little cross-reactivity with some of related steroids.

Related Compounds

  • 17-Beta-Estradiol...

Related Articles:

Sulfotransferase 2A1 forms estradiol-17-sulfate and celecoxib switches the dominant product from estradiol-3-sulfate to estradiol-17-sulfate.

2005-09-01

[J. Steroid Biochem. Mol. Biol. 96(5) , 367-74, (2005)]

Steroid sulphate secretion by the human ovary in vitro: influence of gonadotrophins, cyclofenil and clomiphene.

1980-12-01

[J. Steroid Biochem. 13(12) , 1401-6, (1980)]

[Evidence, in vitro, of estrone and estradiol-17 beta 3-sulfoconjugate hydrolysis in the uterus of pregnant guinea pigs].

1981-03-02

[C. R. Seances. Acad. Sci. III. 292(9) , 609-12, (1981)]

Genomics of estradiol-3-sulfate action in the ovine fetal hypothalamus.

2012-07-03

[Physiol. Genomics 44(13) , 669-77, (2012)]

Fetal hypothalamus-pituitary-adrenal responses to estradiol sulfate.

2011-12-01

[Endocrinology 152(12) , 4966-73, (2011)]

More Articles...