Molecular Membrane Biology 2004-01-01

The supramolecular structure of the GPCR rhodopsin in solution and native disc membranes.

Kitaru Suda, Slawomir Filipek, Krzysztof Palczewski, Andreas Engel, Dimitrios Fotiadis

Index: Mol. Membr. Biol. 21(6) , 435-46, (2004)

Full Text: HTML

Abstract

Rhodopsin, the prototypical G-protein-coupled receptor, which is densely packed in the disc membranes of rod outer segments, was proposed to function as a monomer. However, a growing body of evidence indicates dimerization and oligomerization of numerous G-protein-coupled receptors, and atomic force microscopy images revealed rows of rhodopsin dimers in murine disc membranes. In this work we demonstrate by electron microscopy of negatively stained samples, blue native- and sodium dodecyl sulphate-polyacrylamide gel electrophoresis, chemical crosslinking, and by proteolysis that native bovine rhodopsin exists mainly as dimers and higher oligomers. These results corroborate the recent findings from atomic force microscopy and molecular modeling on the supramolecular structure and packing arrangement of murine rhodopsin dimers.

Related Compounds

  • DSP Crosslinker

Related Articles:

Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin.

2015-01-01

[Sensors (Basel.) 15 , 19212-24, (2015)]

Targeting delivery of tocopherol and doxorubicin grafted-chitosan polymeric micelles for cancer therapy: In vitro and in vivo evaluation.

2015-09-01

[Colloids Surf. B Biointerfaces 133 , 254-62, (2015)]

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.

2014-12-02

[Sci. Signal. 7(354) , rs7, (2014)]

Biothiol-triggered, self-disassembled silica nanobeads for intracellular drug delivery.

2015-09-01

[Acta Biomater. 23 , 263-70, (2015)]

Pre-fertilization zona pellucida hardening by different cross-linkers affects IVF in pigs and cattle and improves embryo production in pigs.

2009-05-01

[Reproduction 137(5) , 803-12, (2009)]

More Articles...