Derivatization of isolated endogenous butyrobetaine with 4'-bromophenacyl trifluoromethanesulfonate followed by high-performance liquid chromatography.

S Krahenbuhl, P E Minkler, C L Hoppel

Index: J. Chromatogr. A. 573(1) , 3-10, (1992)

Full Text: HTML

Abstract

A method for the isolation and chromatography of butyrobetaine from plasma, urine, and liver is described. The recovery of [3H-methyl]butyrobetaine from spiked biological samples was from 76-80%. Spiked samples then were derivatized with 4'-bromophenacyl trifluoromethanesulfonate and the butyrobetaine 4'-bromophenacyl ester was isolated by high-performance liquid chromatography (HPLC). Radioactivity eluted in a single peak which co-chromatographed with authentic butyrobetaine 4'-bromophenacyl ester. Two identical liver specimens were treated according to this isolation procedure. Prior to derivatization, one specimen was treated with butyrobetaine hydroxylase. After derivatization, there was no butyrobetaine 4'-bromophenacyl ester peak in the specimen treated with butyrobetaine hydroxylase. The HPLC detection sensitivity to butyrobetaine 4'-bromophenacyl ester was 1 pmol injected with a signal-to-noise greater than 2:1.

Related Compounds

  • 4-BROMOPHENAC...

Related Articles:

Analysis of carnitine and acylcarnitines in urine by capillary electrophoresis.

2000-10-20

[J. Chromatogr. A. 895 , 309, (2000)]

Determination of free carnitine and 'total' carnitine in human urine: derivatization with 4'-bromophenacyl trifluoromethanesulfonate and high performance liquid chromatography.

1992-11-16

[Clin. Chim. Acta 212 , 55, (1992)]

Quantification of carnitine and specific acylcarnitines by high-performance liquid chromatography: application to normal human urine and urine from patients with methylmalonic aciduria, isovaleric acidemia or medium-chain acyl-CoA dehydrogenase deficiency.

[J. Chromatogr. A. 613(2) , 203-21, (1993)]

Derivatization of carboxylic acids by reaction with 4'-bromophenacyl trifluoromethanesulfonate prior to determination by high-performance liquid chromatography.

[J. Chromatogr. A. 299(2) , 365-76, (1984)]

Strategy for the isolation, derivatization, chromatographic separation, and detection of carnitine and acylcarnitines.

2005-03-01

[Anal. Chem. 77(5) , 1448-57, (2005)]

More Articles...