Biotechnology Letters 2011-11-01

Over-expression of chloroperoxidase in Caldariomyces fumago.

Markus Buchhaupt, Kristina Ehrich, Sonja Hüttmann, Jan Guder, Jens Schrader

Index: Biotechnol. Lett. 33(11) , 2225-31, (2011)

Full Text: HTML

Abstract

The filamentous fungus Caldariomyces fumago secretes a chloroperoxidase (CPO). To increase its production, we integrated a CPO-expression cassette into the non-transcribed spacer regions of the rDNA in C. fumago. One strain was obtained that had twice the CPO activity when grown in shake-flask and bioreactor compared to the wild-type. The highest CPO activity from the bioreactor cultivation was 3,236 U ml(-1). This is the highest value reported so far.

Related Compounds

  • Chloroperoxidase,...

Related Articles:

Use of Cl and C isotopic fractionation to identify degradation and sources of polychlorinated phenols: mechanistic study and field application.

2013-01-15

[Environ. Sci. Technol. 47(2) , 790-7, (2013)]

Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors.

2009-11-21

[Org. Biomol. Chem. 7 , 4604-4610, (2009)]

Catalytic performance and thermostability of chloroperoxidase in reverse micelle: achievement of a catalytically favorable enzyme conformation.

2011-06-01

[J. Ind. Microbiol. Biotechnol. 38(6) , 717-24, (2011)]

Enzymatic modification of chitosan with quercetin and its application as antioxidant edible films.

2012-01-01

[Prikl. Biokhim. Mikrobiol. 48(2) , 175-82, (2012)]

Detoxification of sulfur mustard by enzyme-catalyzed oxidation using chloroperoxidase.

2013-10-10

[Enzyme Microb. Technol. 53(5) , 295-301, (2013)]

More Articles...