Journal of AOAC International 2013-01-01

High-performance liquid chromatographic determination of Ethamsylate in urine and tablets.

Alaa El-Gindy, Mohammad Wafaa Nassar, Khalid Abdel-Salam Attia, Hamed Hamed Abu-Seada, Mahmoud El-Ghandour

Index: J. AOAC Int. 96(4) , 730-6, (2013)

Full Text: HTML

Abstract

A simple, selective, sensitive, accurate, and precise method was developed for determination of ethamsylate (ET) in human urine and in ET tablets using RP-HPLC. The method uses a C18 (5 pm particle size) column at ambient temperature with the mobile phase 14.7 mM potassium dihydrogen phosphate (pH 4.6)-8.15 mM tetraheptylammonium bromide in acetonitrile (50 + 50, v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 300 nm, based on peak area with a linear calibration curve in the concentration range of 0.1-100 microg/mL. The proposed method was applied for the determination of the urinary excretion pattern of ET as the cumulative amounts excreted have been calculated without pretreatment of urine samples. The proposed method was completely validated according to U.S. Food and Drug administration guidelines.

Related Compounds

  • Etamsylate

Related Articles:

Application and equivalence assessment for determining ethamsylate by using potassium ferricyanide as spectroscopic probe reagent.

2010-01-01

[Anal. Sci. 26(11) , 1163-7, (2010)]

Simultaneous determination of aminomethylbenzoic acid, cefminox sodium and etamsylate in human urine by capillary electrophoresis.

2010-07-01

[J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 878(21) , 1899-903, (2010)]

A novel visible spectrophotometric method for the determination of ethamsylate in pharmaceutical preparations and biological samples.

2010-03-01

[Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 75(3) , 1013-7, (2010)]

Short-term efficacy of intravitreal dobesilate in central serous chorioretinopathy.

2012-01-01

[Eur. J. Med. Res. 17 , 22, (2012)]

Conventional and first derivative synchronous fluorometric determination of ethamsylate in pharmaceutical preparations and biological fluids. Application to stability studies.

2011-07-01

[J. Fluoresc. 21(4) , 1371-84, (2011)]

More Articles...