Blood cells 1993-01-01

Fluorescence of eosinophil leukocyte granules induced by the fluorogenic reagent 2-methoxy-2,4-diphenyl-3 (2H)-furanone.

J C Stockert, C I Trigoso

Index: Blood Cells 19(2) , 423-30; discussion 431-3, (1993)

Full Text: HTML

Abstract

The fluorogenic reagent MDPF forms highly fluorescent products specifically with primary amino groups (e.g., from lysine). Although widely used in analytical biochemistry, MDPF has infrequently been employed in cytochemical techniques for proteins. In this work, we describe the application of MDPF in fluorescence microscopy studies of horse, rat, and human blood cells. Under ultraviolet excitation, MDPF selectively induces an intense white-blue fluorescence reaction in the granules of eosinophil leukocytes, which contain strongly basic proteins. The advantages of MDPF for identification to reveal protein components in hematological cytochemistry are briefly discussed.

Related Compounds

  • 2-Methoxy-2,4-diph...

Related Articles:

Proteolytic activity and cytokine up-regulation by non-albicans Candida albicans.

2015-05-01

[Arch. Microbiol. 197(4) , 533-7, (2015)]

Fluorescent labeling of proteins with nile red and 2-methoxy-2,4-diphenyl-3(2H)-furanone: physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots.

2001-03-01

[Electrophoresis 22(5) , 874-80, (2001)]

Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in the first-dimensional isoelectric focusing gel with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone.

1988-07-01

[Electrophoresis 9(7) , 330-9, (1988)]

Endocytosis and exocytosis of protein in capillary endothelium.

1984-08-01

[J. Cell Physiol. 120(2) , 157-62, (1984)]

A fluorescent screening assay for collagenase using collagen labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone.

1984-08-01

[Anal. Biochem. 140(2) , 490-4, (1984)]

More Articles...