Histochemistry 1983-01-01

Cytochemical demonstrations of protease in human peripheral blood cells by use of new alpha-naphthyl ester substrates.

T Honda, Y Hitomi, M Niinobe, S Fujii

Index: Histochemistry 77(3) , 299-302, (1983)

Full Text: HTML

Abstract

The proteases of human leukocytes were cytochemically studied by use of new alpha-naphthyl esters, tosyl-L-lysine-alpha-naphthyl ester (TLNE) and acetyl-L-tyrosine-alpha-naphthyl ester (ATNE). The hydrolytic activities were strong only in neutrophils, with both substrates. They were inhibited completely by DFP and chymostatin, but not by leupeptin and iodoacetate. These results indicate that chymotrypsin-like enzyme(s), capable of hydrolyzing both substrates, exist in neutrophils.

Related Compounds

  • 1-Naphthyl butyrat...

Related Articles:

Esterase isoenzymes and insecticide resistance in Frankliniella occidentalis populations from the south-east region of Spain.

2008-12-01

[Pest Manag. Sci. 64(12) , 1258-66, (2008)]

Ultrastructural cytochemistry of non-specific esterase in murine peritoneal macrophages.

1989-05-01

[Histochem. J. 21(5) , 301-8, (1989)]

Recommended methods for cytological procedures in haematology. International Committee for Standardization in Haematology (ICSH).

1985-01-01

[Clin. Lab. Haematol. 7(1) , 55-74, (1985)]

Single incubation double esterase cytochemical reaction using a single coupling reagent.

1984-10-01

[J. Clin. Pathol. 37(10) , 1187-90, (1984)]

Carboxylesterase activity, cDNA sequence, and gene expression in malathion susceptible and resistant strains of the cotton aphid,Aphis gossypii

2009-01-01

[Comp. Biochem. Physiol. B Biochem. Mol. Biol. 152(3) , 266-70, (2009)]

More Articles...