Bioorganic & Medicinal Chemistry Letters 2009-07-15

One plasmid selection system for the rapid evolution of aminoacyl-tRNA synthetases.

Charles E Melançon, Peter G Schultz

Index: Bioorg. Med. Chem. Lett. 19 , 3845-3847, (2009)

Full Text: HTML

Abstract

We have developed a rapid, straightforward, one plasmid dual positive/negative selection system for the evolution of aminoacyl-tRNA synthetases with altered specificities in Escherichia coli. This system utilizes an amber stop codon containing chloramphenicol acetyltransferase/uracil phosphoribosyltransferase fusion gene. We demonstrate the utility of the system by identifying a variant of the Methanococcus jannaschii tyrosyl synthetase from a library of 10(9) variants that selectively incorporates para-iodophenylalanine in response to an amber stop codon.

Related Compounds

  • H-Phe(4-I)-OH

Related Articles:

A nonradioactive approach to investigate the metabolism of therapeutic peptides by tagging with 127i and using inductively-coupled plasma mass spectrometry analysis.

2015-01-01

[Drug Metab. Dispos. 43(1) , 17-26, (2014)]

An expanded eukaryotic genetic code.

2003-08-15

[Science 301(5635) , 964-7, (2003)]

p-[123I]iodo-L-phenylalanine for detection of pancreatic cancer: basic investigations of the uptake characteristics in primary human pancreatic tumour cells and evaluation in in vivo models of human pancreatic adenocarcinoma.

2004-04-01

[Eur. J. Nucl. Med. Mol. Imaging 31(4) , 532-41, (2004)]

Validation of brain tumour imaging with p-[123I]iodo-L-phenylalanine and SPECT.

2005-09-01

[Eur. J. Nucl. Med. Mol. Imaging 32(9) , 1041-9, (2005)]

Initial evaluation of the feasibility of single photon emission tomography with p-[123 I]iodo-L-phenylalanine for routine brain tumour imaging.

2002-02-01

[Nucl. Med. Commun. 23(2) , 121-30, (2002)]

More Articles...