Journal of the American Chemical Society 2005-07-27

Artificial polypeptide scaffold for protein immobilization.

Kechun Zhang, Michael R Diehl, David A Tirrell

Index: J. Am. Chem. Soc. 127(29) , 10136-7, (2005)

Full Text: HTML

Abstract

An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.

Related Compounds

  • octyltrichlorosila...

Related Articles:

Supports for reverse-phase high-performance liquid chromatography of large proteins.

1980-05-01

[Anal. Biochem. 104(1) , 153-9, (1980)]

Cloning SU8 silicon masters using epoxy resins to increase feature replicability and production for cell culture devices.

2015-05-01

[Biomicrofluidics 9 , 036502, (2015)]

Multimodal underwater adsorption of oxide nanoparticles on catechol-based polymer nanosheets.

2016-03-21

[Nanoscale 8 , 5912-9, (2016)]

Anodized aluminum oxide thin films for room-temperature-processed, flexible, low-voltage organic non-volatile memory elements with excellent charge retention.

2011-11-09

[Adv. Mater. 23(42) , 4892-6, (2011)]

X-ray scattering study of thin films of poly(2,5-bis(3-alkylthiophen-2-yl)thieno[3,2-b]thiophene).

2007-03-21

[J. Am. Chem. Soc. 129(11) , 3226-37, (2007)]

More Articles...