British journal of industrial medicine 1981-08-01

Thin-layer chromatography of hippuric and m-methylhippuric acid in urine after mixed exposure to toluene and xylene.

G Bieniek, T Wilczok

Index: Br. J. Ind. Med. 38(3) , 304-6, (1981)

Full Text: HTML

Abstract

The separation of hippuric and m-methylhippuric acid as toluene and m-xylene metabolites present in urine of people exposed simultaneously to toluene and xylene is described. Chloroform was used for hippuric and m-methylhippuric acid extraction. Satisfactory separation of these metabolites was obtained on TLC plates covered with silica gels and developed in chloroform acetic acid-water (4:1:1);p-dimethylaminobenzaldehyde in acetic acid anhydride was applied to develop the colour. The sensitivity of the method was 6 micrograms hippuric acid per 1 ml urine and recovery was 100% (+/- 1).

Related Compounds

  • 4-Methylhippuric a...

Related Articles:

Development of a multi-residue method in a fetal matrix: analysis of meconium.

2014-12-01

[Anal. Bioanal. Chem 406(30) , 7785-97, (2014)]

Development of liquid chromatography methods coupled to mass spectrometry for the analysis of substances with a wide variety of polarity in meconium.

2015-06-01

[Talanta 138 , 231-9, (2015)]

[Simultaneous determination of urinary hippuric acid and o-, m-, p-methylhippuric acids with liquid chromatography].

[J. Chromatogr. A. 382 , 275-9, (1986)]

Simultaneous determination by gas chromatography of the major metabolites in urine of toluene, xylenes and styrene.

1978-02-01

[Br. J. Ind. Med. 35(1) , 56-60, (1978)]

Quantitative analysis of urinary glycine conjugates by high performance liquid chromatography: excretion of hippuric acid and methylhippuric acids in the urine of subjects exposed to vapours of toluene and xylenes.

1986-01-01

[Int. Arch. Occup. Environ. Health 58(2) , 121-9, (1986)]

More Articles...