PNAS 1988-06-01

Specific and stable labeling of antibodies with technetium-99m with a diamide dithiolate chelating agent.

A R Fritzberg, P G Abrams, P L Beaumier, S Kasina, A C Morgan, T N Rao, J M Reno, J A Sanderson, A Srinivasan, D S Wilbur

Index: Proc. Natl. Acad. Sci. U. S. A. 85(11) , 4025-9, (1988)

Full Text: HTML

Abstract

Technetium-99m labeling of antibodies has been suboptimal because of low affinity adventitious binding, nonspecific labeling, and loss of immunoreactivity. The diamide dithiolate ligand system (N2S2) forms highly stable, well-defined tetradentate complexes with Tc(V). Antibodies and their fragments have been labeled by conjugation of preformed 99mTc-4,5-bis(thioacetamido)pentanoate active ester to protein amine groups to give a chemically known 99mTc-N2S2 complex covalently linked to antibody. Evaluations of the 99mTc-N2S2-bound antibodies and their fragments have shown high stability and retained immunoreactivity.

Related Compounds

  • 2,3,5,6-Tetrafluor...

Related Articles:

Atmospheric pressure chemical ionization of fluorinated phenols in atmospheric pressure chemical ionization mass spectrometry, tandem mass spectrometry, and ion mobility spectrometry.

1999-11-01

[J. Am. Soc. Mass Spectrom. 10(11) , 1157-65, (1999)]

Cytochrome P450-mediated oxidation of pentafluorophenol to tetrafluorobenzoquinone as the primary reaction product.

1993-01-01

[Chem. Res. Toxicol. 6(5) , 674-80, (1993)]

Synthesis and radioiodination of N-Boc-p-(tri-n-butylstannyl)-L-phenylalanine tetrafluorophenyl ester: preparation of a radiolabeled phenylalanine derivative for peptide synthesis.

1993-01-01

[Bioconjug. Chem. 4(6) , 574-80, (1993)]

Effect of substrate and pH on the oxidative half-reaction of phenol hydroxylase.

1985-06-01

[J. Biol. Chem. 260(10) , 5998-6005, (1985)]

More Articles...