The hydroxy-naphthoquinone lapachol arrests mycobacterial growth and immunomodulates host macrophages

Renato A.S. Oliveira, Eulalia Azevedo-Ximenes, Roberto Luzzati, Rodolfo C. Garcia, Renato A.S. Oliveira, Eulalia Azevedo-Ximenes, Roberto Luzzati, Rodolfo C. Garcia, Renato A.S. Oliveira, Eulalia Azevedo-Ximenes, Roberto Luzzati, Rodolfo C. Garcia

Index: Int. Immunopharmacol. 10(11) , 1463-73, (2010)

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Abstract

The present study reports the anti-mycobacterial activity of 2-hydroxy-3-(3-methyl-2-butenyl)-1,4-naphthoquinone (lapachol) as well as its influence on macrophage functions. Lapachol (L) did not induce apoptosis/necrosis of THP-1 macrophages at ≤ 32 μg/mL. Mycobacterium avium liquid growth was arrested by ≥ 32 μg/mL and intra-macrophage proliferation by ≥ 16 μg/mL lapachol. The main immuno-modulatory effects of lapachol observed were an up-regulation of interferon-γ-receptor 1 (IFN-γR1) and major histocompatibility complex class II (MHCII) surface expression, and a marked inhibition of IL-10 secretion. Lapachol did not affect resting, IFN-γ- or toll-like receptor 2 (TLR2)-induced levels of oxygen and nitrogen metabolism key proteins nor the TLR2-mediated secretion of TNF-α, nor induced either oxidative or endoplasmic reticulum (ER) stress. Lapachol inhibited the surface expression of the co-stimulatory molecule CD86 but not that of CD80 and CD83. The results obtained indicate that the substituted naphthoquinone lapachol exhibits an anti-mycobacterial activity that is more efficient intra- than extra-cellularly, and exerts immuno-modulatory effects some of which may enhance the capacity of the host cell to control mycobacterial growth. The immune-modulatory action of lapachol could contribute to its more efficient intra-macrophage anti-mycobacterial activity.