Steroids 1991-12-01

Fluorometric labeling of tetrahydroprogesterones.

J C Touchstone, X Fang

Index: Steroids 56(12) , 601-5, (1991)

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Abstract

7-Diethylaminocourmarin-3-carbohydrazide was used to label the ketone group of the tetrahydroprogesterones to form fluorescent derivatives with high sensitivity. The four isomeric 3-hydroxypregnanes separated readily on high-performance, thin-layer chromatography after derivatization. This separation was not possible with the underivatized isomers. Standards and steroids from biologic mixtures were separated and showed similar characteristics. The methods used are described.

Related Compounds

  • 7-Diethylamino-2-...

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