Analytical Biochemistry 2011-01-01

High-throughput analysis of hexosamine using a colorimetric method.

Yu-Shen Cheng, John Labavitch, Jean S VanderGheynst

Index: Anal. Biochem. 408(1) , 160-2, (2011)

Full Text: HTML

Abstract

A 96-well plate method was developed for analysis of total hexosamine content in biological samples. Four hexosamine monomer derivatives-glucosamine hydrochloride, glucosamine sulfate, galactosamine hydrochloride, and mannosamine hydrochloride-were examined for the linearity of their spectra in the concentration range specified in the assay. The hexosamine concentration analysis range was linear from 0.1 to 1 mM. The quantification of hexosamines from chitin and chitosan upon acid hydrolysis was also tested. Accurate quantification of glucosamine content in chitin and chitosan with different molecular sizes and degrees of acetylation was demonstrated using the new method.Copyright © 2010 Elsevier Inc. All rights reserved.

Related Compounds

  • D-Mannosamine hyd...

Related Articles:

Sialidases as regulators of bioengineered cellular surfaces.

2015-07-01

[Glycobiology 25 , 784-91, (2015)]

Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids.

2015-12-01

[Anal. Bioanal. Chem 407 , 8945-58, (2015)]

Conjunctival vaccination against Brucella ovis in mice with mannosylated nanoparticles.

2012-09-28

[J. Control. Release 162(3) , 553-60, (2012)]

A new route for the synthesis of Streptococcus pneumoniae 19F and 19A capsular polysaccharide fragments avoiding the beta-mannosamine glycosylation step.

2009-08-17

[Carbohydr. Res. 344(12) , 1442-8, (2009)]

Efficient metabolic engineering of GM3 on tumor cells by N-phenylacetyl-D-mannosamine.

2006-03-21

[Biochemistry 45(11) , 3733-9, (2006)]

More Articles...