Peptide research 1996-01-01

Improvements to the TMSBr method of peptide resin deprotection and cleavage: application to large peptides.

J T Sparrow, O D Monera

Index: Pept. Res. 9(5) , 218-22, (1996)

Full Text: HTML

Abstract

The original trimethylsilyl bromide (TMSBr) method of peptide resin deprotection and cleavage has been modified for convenience and applicability to larger peptides. Equal amounts of a 66-residue test peptide resin were cleaved by the standard hydrogen fluoride (HF) procedure, the original TMSBr method and the modified TMSBr method. The peptide profile from the original TMSBr cleavage procedure showed multiple products and a lower overall yield. In contrast, the modified TMSBr procedure gave high yields of crude products comparable in purity to those obtained by HF cleavage.

Related Compounds

  • Bromo(trimethyl)si...

Related Articles:

EPR and NMR spectroscopies provide input on the coordination of Cu(I) and Ag(I) to a disordered methionine segment.

2015-06-01

[J. Biol. Inorg. Chem. 20 , 719-27, (2015)]

Position and length of fatty acids strongly affect receptor selectivity pattern of human pancreatic polypeptide analogues.

2014-11-01

[ChemMedChem 9(11) , 2463-74, (2014)]

Reduced vascular endothelial growth factor and capillary density in the occipital cortex in dementia with Lewy bodies.

2014-07-01

[Brain Pathol. 24(4) , 334-43, (2014)]

Two-step hard acid deprotection/cleavage procedure for solid phase peptide synthesis.

1991-02-01

[Int. J. Pept. Protein Res. 37(2) , 145-52, (1991)]

The chemoselective and efficient deprotection of silyl ethers using trimethylsilyl bromide.

2008-06-21

[Org. Biomol. Chem. 6(12) , 2168-72, (2008)]

More Articles...