The Histochemical Journal 1984-05-01

A direct-colouring, metal precipitation method for the demonstration of arylsulphatases A and B.

S Partanen

Index: Histochem. J. 16(5) , 501-6, (1984)

Full Text: HTML

Abstract

A new, direct-colouring, metal precipitation method for the light microscopical demonstration of arylsulphatases A and B is described. It is based on the reducing capacity of nitrocatechol liberated by arylsulphatases from p-nitrocatechol sulphate. The reaction is carried out in Karnovsky-Roots' copper ferricyanide incubation mixture at pH 5.0 or 5.5; the nitrocatechol liberated reduces ferricyanide to ferrocyanide, which in turn forms a brown precipitate with copper that indicates enzyme activity. Enzyme activity was localized in cytoplasmic particles compatible with a lysosomal localization of the enzyme. The histochemical reaction was inhibited by phosphate, which has been shown to inhibit arylsulphatases A and B in biochemical determinations. The method described is technically simple, and sections can be mounted in synthetic resin after dehydration.

Related Compounds

  • Dipotassium 5-nitr...

Related Articles:

Arylsulfatase A bound to poly(butyl cyanoacrylate) nanoparticles for enzyme replacement therapy--physicochemical evaluation.

2014-07-01

[Pharmazie 69(7) , 518-24, (2014)]

Investigation of catalytic loop structure, dynamics, and function relationship of Yersinia protein tyrosine phosphatase by temperature-jump relaxation spectroscopy and X-ray structural determination.

2012-05-31

[J. Phys. Chem. B 116(21) , 6166-76, (2012)]

Isolation and characterization of rat hepatic ascorbic acid-2-sulfatases.

1987-01-01

[Enzyme 37(3) , 134-40, (1987)]

Chemical characterization and substrate specificity of rabbit liver aryl sulfatase A.

1980-07-10

[Biochim. Biophys. Acta 614(1) , 92-101, (1980)]

Ultrastructural localization of Trypanosoma cruzi lysosomes by aryl sulphatase cytochemistry.

2007-01-01

[Micron 38(3) , 252-6, (2007)]

More Articles...