Pharmazie 2008-01-01

Quantitative determination of vasicine and vasicinone in Adhatoda vasica by high performance capillary electrophoresis.

B Avula, S Begum, S Ahmed, M I Choudhary, I A Khan

Index: Pharmazie 63(1) , 20-2, (2008)

Full Text: HTML

Abstract

A new method of capillary electrophoresis was developed for the quantitative determination of vasicine and vasicinone from Adhatoda vasica (L.) Nees. The electrophoretic separation was performed using a 47 cm x 50 microm ID (38.5 cm effective length) fused silica capillary. The samples were injected by pressure for 3 s at 50 mbar and the running voltage was 19 kV at the injector end of the capillary. The capillary temperature was maintained at 40 degrees C. The separation of the two alkaloids has been achieved within 11 min with good repeatability. The method was validated in terms of reproducibility, linearity, accuracy and applied for the quantitative determination of vasicine and vasicinone in A. vasica plant samples/extracts. Parameters affecting the resolution such as pH, temperature, organic modifier, buffer concentration and capillary dimensions were reported.

Related Compounds

  • vasicine

Related Articles:

Potentiation of oxytocin evoked responses by (+) sotalol deoxysotalol and Vasicine HCl on isolated rat and rabbit uterus.

1982-12-01

[Indian J. Med. Res. 76 Suppl , 107-14, (1982)]

Natural products as templates for bioactive compound libraries: part 2. Novel modifications of vasicine (peganine) core via efficient and regioselective generation of 3-lithiodeoxyvasicine and its stereoselective addition to aliphatic ketones section sign.

2006-07-20

[Nat. Prod. Res. 20(9) , 871-81, (2006)]

Formulation and pharmacokinetic evaluation of hard gelatin capsule encapsulating lyophilized Vasa Swaras for improved stability and oral bioavailability of vasicine.

2011-04-01

[Fitoterapia 82(3) , 446-53, (2011)]

Thrombopoietic activity of vasicine hydrochloride.

1982-09-01

[Indian J. Exp. Biol. 20(9) , 704-9, (1982)]

Detection and confirmation of alkaloids in leaves of Justicia adhatoda and bioinformatics approach to elicit its anti-tuberculosis activity.

2012-11-01

[Appl. Biochem. Biotechnol. 168(5) , 980-90, (2012)]

More Articles...