A quenched-flow technique for the measurement of glucose influx into human red blood cells.
A G Lowe, A R Walmsley
Index: Anal. Biochem. 144(2) , 385-9, (1985)
Full Text: HTML
Abstract
A quenched-flow apparatus is described and applied to measurements of the hydrolysis of 2,4-dinitrophenyl acetate by sodium hydroxide and the entry of D-[U-14C]glucose into human red blood cells at 37 degrees C. Glucose influx into red cells was a saturable process obeying Michaelis-Menten kinetics with a Km for glucose of 6.6 +/- 0.61 mM and a maximum rate for glucose entry under "zero trans" conditions of 20.7 +/- 0.76 mmol (L cell water)-1 s-1. The technique used requires only readily available laboratory equipment and should be easily adaptable to the study of other rapid transport processes.
Related Compounds
Related Articles:
1992-09-01
[Eur. J. Biochem. 208(2) , 289-94, (1992)]
Synthesis of a parabactin photoaffinity label. Bergeron RJ, et al.
[J. Org. Chem. 52(1) , 144-49, (1987)]
The coupling of diazonium salts with aliphatic carbon atoms. Parmerter SM.
[Org. React. , (1959)]
2007-10-10
[J. Am. Chem. Soc. 129(40) , 12082-3, (2007)]
1980-07-15
[Anal. Biochem. 106(1) , 73-7, (1980)]