Biochemical and Biophysical Research Communications 1986-12-30

The antilipolytic effect of insulin in human adipocytes requires activation of the phosphodiesterase.

P Lönnroth, U Smith

Index: Biochem. Biophys. Res. Commun. 141(3) , 1157-61, (1986)

Full Text: HTML

Abstract

Human fat cells were incubated with two different cAMP analogues, 8-bromocAMP and 6N-monobutyrylcAMP. The former analogue is an excellent substrate for the phosphodiesterase while the latter is resistant to hydrolysis. In the presence of adenosine deaminase, isoproterenol (10(-6)M) stimulated lipolysis 8-10 fold which was similar to the effect exerted by the cAMP analogues. Basal lipolysis and lipolysis activated by 6N-monobutyrylcAMP was not inhibited by insulin even at high concentrations, whereas the effect of 8-bromocAMP was virtually completely inhibited. This effect of insulin was completely prevented by the addition of IBMX. Thus, activation of phosphodiesterase by insulin is necessary to elicit the antilipolytic effect in human adipocytes.

Related Compounds

  • N6-Monobutyrylade...

Related Articles:

Protein kinase A and B-Raf mediate extracellular signal-regulated kinase activation by thyrotropin.

2009-11-01

[Mol. Pharmacol. 76 , 1123-1129, (2009)]

Cryptic receptors for insulin-like growth factor II in the plasma membrane of rat adipocytes--a possible link to cellular insulin resistance.

1996-06-13

[Biochim. Biophys. Acta 1282(1) , 57-62, (1996)]

Expression of aquaporin 8 and its up-regulation by cyclic adenosine monophosphate in human WISH cells.

2003-04-01

[Am. J. Obstet. Gynecol. 188(4) , 997-1001, (2003)]

Abnormal adenosine 3'.5'-monophosphate stimulation of renal 1,25-dihydroxyvitamin D production in hyp mice: evidence that 25-hydroxyvitamin D-1 alpha-hydroxylase dysfunction results from aberrant intracellular function.

1989-03-01

[Endocrinology 124(3) , 1184-9, (1989)]

Acquisition of a transcriptionally permissive state during the 1-cell stage of mouse embryogenesis.

1992-02-01

[Dev. Biol. 149(2) , 457-62, (1992)]

More Articles...