Isolation of the intermediate filament protein vimentin by chromatofocusing.

H Bloemendal, M Willemsen, G Groenewoud, P Oomen

Index: FEBS Lett. 180 , 181, (1985)

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Abstract

A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M urea and at 22 degrees C is 4.9. Electrophoretic mobility on one- and two-dimensional polyacrylamide gels, solubility in 6 M urea and amino acid composition were used for identification.