Methods in Molecular Biology 2011-01-01

DNA methyltransferase assays.

Renata Z Jurkowska, Alexandre Ceccaldi, Yingying Zhang, Paola B Arimondo, Albert Jeltsch

Index: Methods Mol. Biol. 791 , 157-177, (2011)

Full Text: HTML

Abstract

DNA methyltransferases are important enzymes and their inhibition has many potential applications. The investigation of DNA methyltransferases as well as screening for potential inhibitors requires specialized enzyme assays. In this chapter, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using radioactively labeled AdoMet and biotinylated DNA substrates that is ideal for enzymatic characterization of these enzymes. (2) An assay using bisulfite conversion of in vitro methylated DNA that is ideal to determine details of the methylation pattern introduced by DNA-(cytosine C5)-methyltransferases. (3) A novel fluorescence-coupled, restriction-based assay suitable for high-throughput screening of DNA methyltransferase inhibitors.

Related Compounds

  • 5'-[[(3S)-3-AMIN...

Related Articles:

In vitro and in vivo biotransformation of WMS-1410, a potent GluN2B selective NMDA receptor antagonist.

2014-06-01

[J. Pharm. Biomed. Anal. 94 , 36-44, (2014)]

Structure and reaction mechanism of phosphoethanolamine methyltransferase from the malaria parasite Plasmodium falciparum: an antiparasitic drug target.

2012-01-06

[J. Biol. Chem. 287 , 1426-1434, (2012)]

DNA methyltransferases: mechanistic models derived from kinetic analysis.

2012-01-01

[Crit. Rev. Biochem. Mol. Biol. 47(2) , 97-193, (2012)]

Radical AdoMet enzymes in complex metal cluster biosynthesis.

2012-11-01

[Biochim. Biophys. Acta 1824(11) , 1254-63, (2012)]

Enzymatic methyl transfer: role of an active site residue in generating active site compaction that correlates with catalytic efficiency.

2011-11-02

[J. Am. Chem. Soc. 133 , 17134-17137, (2011)]

More Articles...