Synthesis and Characterization of Fluorenone-, Anthraquinone-, and Phenothiazine-Labeled Oligodeoxynucleotides: 5'-Probes for DNA Redox Chemistry

MT Tierney, MW Grinstaff

Index: Tierney, Mark T.; Grinstaff, Mark W. Journal of Organic Chemistry, 2000 , vol. 65, # 17 p. 5355 - 5359

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Citation Number: 88

Abstract

A facile and automated procedure for the synthesis of oligodeoxynucleotides possessing derivatives of 9-fluoreneone, 9, 10-anthraquinone, and phenothiazine is described. The phosphoramidite approach is used to attach these redox and spectroscopic probes to the 5'- terminus of oligodeoxynucleotides in high yield (> 95%). Thermal denaturation studies of labeled oligodeoxynucleotides show a slight enhancement in duplex stability relative to ...