Mag-Indo-1 tetrapotassium salt
Modify Date: 2024-01-04 09:36:35
Mag-Indo-1 tetrapotassium salt structure
|
Common Name | Mag-Indo-1 tetrapotassium salt | ||
|---|---|---|---|---|
| CAS Number | 132299-21-9 | Molecular Weight | 594.73700 | |
| Density | N/A | Boiling Point | 840.9ºC at 760mmHg | |
| Molecular Formula | C21H14K4N2O9 | Melting Point | N/A | |
| MSDS | Chinese USA | Flash Point | 462.3ºC | |
Use of Mag-Indo-1 tetrapotassium saltMag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg2+ detection[1]. |
| Name | tetrapotassium,2-[4-[bis(carboxylatomethyl)amino]-3-(carboxylatomethoxy)phenyl]-1H-indole-6-carboxylate |
|---|---|
| Synonym | More Synonyms |
| Description | Mag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg2+ detection[1]. |
|---|---|
| Related Catalog | |
| In Vitro | Mag-Indo-1 tetrapotassium salt can be used to determine the free Mg2+ concentration in the cytoplasmic solutions[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Calibrating solutions containing various concentrations of Mg2+ are prepared by mixing different ratios of the two stock solutions: one contains (mM) 150 KCl, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH), and the other contains 100 MgCl2, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH). 2. The calibration curve is constructed using calibrating solutions containing 1 μM Mag-Indo-1 tetrapotassium salt. 3. The ‘Mg2+’ calibrating solution contained (mM): 130 KCl, 4 EDTA and 5 Hepes (pH 7.2 with KOH). 4. The relationship between the background-corrected value of the fluorescence ratio (R) and the Mg2+ concentration is fitted with the following theoretical equation. [Mg]=K (R-Rmin)/(Rmax-R), where [Mg] is the concentration of free Mg2+ ion, Rmin is the R value at 0 [Mg2+], and Rmax is the R value at saturating Mg2+. The curve fitting gave Rmin= 0.053, Rmax= 1.57, and K= 5.1 mM. Note: the specific curve can refer to the reference. |
| References |
| Boiling Point | 840.9ºC at 760mmHg |
|---|---|
| Molecular Formula | C21H14K4N2O9 |
| Molecular Weight | 594.73700 |
| Flash Point | 462.3ºC |
| Exact Mass | 593.92500 |
| PSA | 188.78000 |
| Vapour Pressure | 5.04E-30mmHg at 25°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
| Mag-indo-1,tetrapotassium salt [Magaptra-1,tetrapotassium salt] |
| Mag-Indo 1 |
| Indo 1 analog tetrapotassium salt |
| Mag-indo-1 tetrapotassium salt |