Journal of Chromatography B 2014-09-01

Profiling of regioisomeric triacylglycerols in edible oils by supercritical fluid chromatography/tandem mass spectrometry.

Jae Won Lee, Toshiharu Nagai, Naohiro Gotoh, Eiichiro Fukusaki, Takeshi Bamba

文献索引：J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 966 , 193-9, (2014)

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摘要

In this study, supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry was applied to the profiling of several regioisomeric triacylglycerols (TAGs). SFC conditions (column, flow rate, modifier) were optimized for the effective separation of TAGs. In the column test, a triacontyl (C30) silica gel reversed-phase column was selected to separate TAG regioisomers. Multiple reaction monitoring was used to selectively quantify each TAG. Then, the method was used to perform detailed characterization of a diverse array of TAGs in palm and canola oils. Seventy TAGs (C46:0-C60:2) of these oils were successfully analyzed as a result, and twenty isomeric TAG pairs were separated well. In particular, this method provided the fast and high resolution separation of six regioisomeric TAG pairs (PPLn/PLnP, PPL/PLP, PPO/POP, SPLn/SLnP, SPO/SOP, SSO/SOS-stearic acid (S, 18:0), oleic acid (O, 18:1), linoleic acid (L, 18:2), linolenic acid (Ln, 18:3), palmitic acid (P, 16:0)) in a short time (50min) as compared to high performance liquid chromatography. We were able to demonstrate the utility of this method for the analysis of regioisomeric TAGs in edible oils. Copyright © 2014 Elsevier B.V. All rights reserved.

相关化合物

  • 甘油
  • 单油酸甘油酯
  • 甲醇
  • 甲酸铵
  • 甘油单油酸酯
  • 1-棕榈酸单甘油酯

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