Journal of Immunology 2015-03-01

MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b.

Xavier Michelet, Salil Garg, Benjamin J Wolf, Amit Tuli, Paola Ricciardi-Castagnoli, Michael B Brenner

文献索引:J. Immunol. 194(5) , 2079-88, (2015)

全文:HTML全文

摘要

Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC II) Ag-presenting molecules in lysosomes. Once MHC II molecules have bound a peptide, the MHC II-peptide complex is delivered to the cell surface for presentation to CD4(+) T cells. Regulation of Ag uptake via macropinocytosis and phagocytosis has been extensively studied, as well as trafficking in early endocytic vesicles notably regulated by the small GTPase Rab5 and its effectors. However, little is known about the regulators of Ag delivery from early endosomes to lysosomal compartments where the proper pH, proteases, MHC II, invariant chain, and HLA-DM reside, awaiting exogenous Ags for loading. In this article, we report the crucial role of the small GTPase ADP-ribosylation factor-like 8b (Arl8b) in MHC II presentation in DCs. We show for the first time, to our knowledge, that Arl8b localizes to MHC II compartments in DCs and regulates formation of MHC II-peptide complexes. Arl8b-silenced DCs display a defect in MHC II-Ag complex formation and its delivery to the cell surface during infection resulting in a defect in T cell recognition. Our results highlight the role of Arl8b as a trafficking regulator of the late stage of complex formation and MHC II presentation in DCs. Copyright © 2015 by The American Association of Immunologists, Inc.


相关化合物

  • 甘油
  • 氯化钠
  • 十二烷基硫酸钠
  • 异丙基-β-D-硫代半...
  • 3,3′,5,5′-四甲基...
  • 丙烯酰胺
  • 5-(((2,5-二氧吡咯...
  • L-谷氨酰胺
  • 4-羟乙基哌嗪乙磺酸
  • 氯化钠-35cl

相关文献:

Salicylic acid signaling controls the maturation and localization of the arabidopsis defense protein ACCELERATED CELL DEATH6.

2014-08-01

[Mol. Plant 7(8) , 1365-83, (2014)]

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

2014-06-02

[J. Exp. Med. 211(6) , 1079-91, (2014)]

Irisin stimulates muscle growth-related genes and regulates adipocyte differentiation and metabolism in humans.

2012-07-01

[Int. J. Obes. 38(12) , 1538-44, (2014)]

Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.

2014-01-01

[PLoS Biol. 12(1) , e1001758, (2014)]

Mechanism of human PTEN localization revealed by heterologous expression in Dictyostelium.

2014-12-11

[Oncogene 33(50) , 5688-96, (2014)]

更多文献...