Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film.

Nedjla Zehani, Philippe Fortgang, Mohamed Saddek Lachgar, Abdoullatif Baraket, Madjid Arab, Sergei V Dzyadevych, Rochdi Kherrat, Nicole Jaffrezic-Renault

文献索引：Biosens. Bioelectron. 74 , 830-5, (2015)

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摘要

A highly sensitive electrochemical biosensor for the detection of Bisphenol A (BPA) in water has been developed by immobilizing tyrosinase onto a diazonium-functionalized boron doped diamond electrode (BDD) modified with multi-walled carbon nanotubes (MWCNTs). The fabricated biosensor exhibits excellent electroactivity towards o-quinone, a product of this enzymatic reaction of BPA oxidation catalyzed by tyrosinase. The developed BPA biosensor displays a large linear range from 0.01 nM to 100 nM, with a detection limit (LOD) of 10 pM. The feasibility of the proposed biosensor has been demonstrated on BPA spiked water river samples. Therefore, it could be a promising and reliable analytical tool for on-site monitoring of BPA in waste water.Copyright © 2015 Elsevier B.V. All rights reserved.