Analytical and Bioanalytical Chemistry 2015-01-01

An integrated microfluidic chip enabling control and spatially resolved monitoring of temperature in micro flow reactors.

Christian Hoera, Stefan Ohla, Zhe Shu, Erik Beckert, Stefan Nagl, Detlev Belder

文献索引:Anal. Bioanal. Chem 407(2) , 387-96, (2015)

全文:HTML全文

摘要

A strength of microfluidic chip laboratories is the rapid heat transfer that, in principle, enables a very homogeneous temperature distribution in chemical processes. In order to exploit this potential, we present an integrated chip system where the temperature is precisely controlled and monitored at the microfluidic channel level. This is realized by integration of a luminescent temperature sensor layer into the fluidic structure together with inkjet-printed micro heating elements. This allows steering of the temperature at the microchannel level and monitoring of the reaction progress simultaneously. A fabrication procedure is presented that allows for straightforward integration of thin polymer layers with optical sensing functionality in microchannels of glass-polydimethylsiloxane (PDMS) chips of only 150 μm width and 29 μm height. Sensor layers consisting of polyacrylonitrile and a temperature-sensitive ruthenium tris-phenanthroline probe with film thicknesses of about 0.5 to 6 μm were generated by combining blade coating and abrasion techniques. Optimal coating procedures were developed and evaluated. The chip-integrated sensor layers were calibrated and investigated with respect to stability, reproducibility, and response times. These microchips allowed observation of temperature in a wide range with a signal change of around 1.6 % per K and a maximum resolution of around 0.07 K. The device is employed to study temperature-controlled continuous micro flow reactions. This is demonstrated exemplarily for the tryptic cleavage of coumarin-modified peptides via fluorescence detection.


相关化合物

  • 硫酸
  • 氟化氢
  • 6-马来酰亚氨基己酸
  • 二甲基亚砜
  • 7-氨基-4-甲基香豆...
  • 甲基丙烯酸丙酯
  • 乙醇钽(V)
  • 8-辛酰氧基芘-1,3,6...

相关文献:

Evaluation of the immune response and protective efficacy of Schistosoma mansoni Cathepsin B in mice using CpG dinucleotides as adjuvant.

2015-01-03

[Vaccine 33(2) , 346-53, (2014)]

Comparison of mcl-Poly(3-hydroxyalkanoates) synthesis by different Pseudomonas putida strains from crude glycerol: citrate accumulates at high titer under PHA-producing conditions.

2014-01-01

[BMC Biotechnol. 14 , 962, (2015)]

Process development for scum to biodiesel conversion.

2015-06-01

[Bioresour. Technol. 185 , 185-93, (2015)]

Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol.

2015-06-01

[Bioresour. Technol. 185 , 49-55, (2015)]

Investigation of the interactions between the EphB2 receptor and SNEW peptide variants.

2014-12-01

[Growth Factors 32(6) , 236-46, (2014)]

更多文献...