Unbinding forces and energies between a siRNA molecule and a dendrimer measured by force spectroscopy.
Andra C Dumitru, Elena T Herruzo, Estrella Rausell, Valentin Ceña, Ricardo Garcia
文献索引:Nanoscale 7 , 20267-76, (2015)
全文:HTML全文
摘要
We have measured the intermolecular forces between small interference RNA (siRNA) and polyamidoamine dendrimers at the single molecular level. A single molecule force spectroscopy approach has been developed to measure the unbinding forces and energies between a siRNA molecule and polyamidoamine dendrimers deposited on a mica surface in a buffer solution. We report three types of unbinding events which are characterized by forces and free unbinding energies, respectively, of 28 pN, 0.709 eV; 38 pN, 0.722 eV; and 50 pN, 0.724 eV. These events reflect different possible electrostatic interactions between the positive charges of one or two dendrimers and the negatively charged phosphate groups of a single siRNA. We have evidence of a high binding affinity of siRNA towards polyamidoamine dendrimers that leads to a 45% probability of measuring specific unbinding events.
相关化合物
相关文献:
2015-01-03
[Vaccine 33(2) , 346-53, (2014)]
2014-01-01
[BMC Biotechnol. 14 , 962, (2015)]
2015-06-01
[Bioresour. Technol. 185 , 185-93, (2015)]
2015-06-01
[Bioresour. Technol. 185 , 49-55, (2015)]
2014-12-01
[Growth Factors 32(6) , 236-46, (2014)]