Biosensors and Bioelectronics 2015-12-15

A nanoplasmonic biosensor for label-free multiplex detection of cancer biomarkers.

Jong Uk Lee, Anh H Nguyen, Sang Jun Sim

文献索引:Biosens. Bioelectron. 74 , 341-6, (2015)

全文:HTML全文

摘要

The development of highly sensitive, selective and multiplex sensors has become an important challenge for disease diagnosis. In this study, we describe a multiplex biosensor for the detection of cancer biomarkers based on unique plasmon response of single gold nanoparticles (AuNPs) and antibody-antigen binding activity. To demonstrate the ability of the plasmon biosensor to detect and quantify cancer biomarkers: a panel of biomarkers, including α-fetoprotein (AFP), carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) was used as a model analyte for multiple detection. A novel and sensitive multiplex biosensor was developed by immobilizing plasmonic nanoparticles in a site-specific manner and functionalized with monoclonal antibodies that recognize the target protein on hydrophilic-hydrophobic patterned glass slide. The proposed multi-analyte biosensor exhibited outstanding selectivity and sensitivity. The limit of detection was determined to be 91 fM, 94 fM and 10 fM for AFP, CEA and PSA from patient-mimicked serum, respectively. Finally, using this sensing strategy, this platform presents an excellent approach for versatile molecular diagnostics in both research and clinical medical fields.Copyright © 2015 Elsevier B.V. All rights reserved.


相关化合物

  • 硫酸
  • 硼氢化钠
  • 八甲基三硅氧烷
  • 六甲基二硅氧烷
  • 过氧化氢
  • 硝酸
  • 硅烷偶联剂KH-550
  • β-巯基乙胺
  • 丙烯酸氯乙酯
  • 1,2-二氯乙烷

相关文献:

Evaluation of the immune response and protective efficacy of Schistosoma mansoni Cathepsin B in mice using CpG dinucleotides as adjuvant.

2015-01-03

[Vaccine 33(2) , 346-53, (2014)]

Comparison of mcl-Poly(3-hydroxyalkanoates) synthesis by different Pseudomonas putida strains from crude glycerol: citrate accumulates at high titer under PHA-producing conditions.

2014-01-01

[BMC Biotechnol. 14 , 962, (2015)]

Process development for scum to biodiesel conversion.

2015-06-01

[Bioresour. Technol. 185 , 185-93, (2015)]

Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol.

2015-06-01

[Bioresour. Technol. 185 , 49-55, (2015)]

Investigation of the interactions between the EphB2 receptor and SNEW peptide variants.

2014-12-01

[Growth Factors 32(6) , 236-46, (2014)]

更多文献...