PNAS 2014-07-08

Shigella reroutes host cell central metabolism to obtain high-flux nutrient supply for vigorous intracellular growth.

David Kentner, Giuseppe Martano, Morgane Callon, Petra Chiquet, Maj Brodmann, Olga Burton, Asa Wahlander, Paolo Nanni, Nathanaël Delmotte, Jonas Grossmann, Julien Limenitakis, Ralph Schlapbach, Patrick Kiefer, Julia A Vorholt, Sebastian Hiller, Dirk Bumann

文献索引:Proc. Natl. Acad. Sci. U. S. A. 111(27) , 9929-34, (2014)

全文:HTML全文

摘要

Shigella flexneri proliferate in infected human epithelial cells at exceptionally high rates. This vigorous growth has important consequences for rapid progression to life-threatening bloody diarrhea, but the underlying metabolic mechanisms remain poorly understood. Here, we used metabolomics, proteomics, and genetic experiments to determine host and Shigella metabolism during infection in a cell culture model. The data suggest that infected host cells maintain largely normal fluxes through glycolytic pathways, but the entire output of these pathways is captured by Shigella, most likely in the form of pyruvate. This striking strategy provides Shigella with an abundant favorable energy source, while preserving host cell ATP generation, energy charge maintenance, and survival, despite ongoing vigorous exploitation. Shigella uses a simple three-step pathway to metabolize pyruvate at high rates with acetate as an excreted waste product. The crucial role of this pathway for Shigella intracellular growth suggests targets for antimicrobial chemotherapy of this devastating disease.


相关化合物

  • 活性炭
  • 丙酮酸
  • D-葡萄糖-13C6,1,2...

相关文献:

Functional characterisation and permeation studies of lyophilised thiolated chitosan xerogels for buccal delivery of insulin.

2014-01-01

[Protein Pept. Lett. 21(11) , 1163-75, (2014)]

Multiplexed microfluidic enzyme assays for simultaneous detection of lipolysis products from adipocytes.

2014-08-01

[Anal. Bioanal. Chem 406(20) , 4851-9, (2014)]

Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models.

2014-08-15

[J. Bacteriol. 196(16) , 3045-57, (2014)]

Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis.

2014-09-15

[J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 967 , 134-8, (2014)]

Acidogenic fermentation of Scenedesmus sp.-AMDD: Comparison of volatile fatty acids yields between mesophilic and thermophilic conditions.

2016-01-01

[Bioresour. Technol. 200 , 624-30, (2015)]

更多文献...