Nanotechnology 2015-09-11

Multivalent linkers for improved covalent binding of oligonucleotides to dye-doped silica nanoparticles.

S M Kelleher, R I Nooney, S P Flynn, E Clancy, M Burke, S Daly, T J Smith, S Daniels, C McDonagh

文献索引:Nanotechnology 26 , 365703, (2015)

全文:HTML全文

摘要

This paper describes the fabrication of oligonucleotide-coated Cy5-doped silica nanoparticles using a combination of multivalent linkers and their use in surface-based DNA sandwich hybridization assays. Dipodal silane is introduced as a means to fabricate amine-coated silica nanoparticles and its advantages compared to monopodal silanes are discussed. The use of dipodal silane in conjunction with three different polymer linkers (oxidized dextran, linear and 8-arm polyethylene glycol (PEG)) to immobilize single-stranded DNA to Cy5-doped nanoparticles is investigated and dynamic light scattering measurements and Fourier transform infrared spectroscopy are used to follow the progression of the functionalization of the nanoparticles. We observe a significant improvement in the binding stability of the single-stranded DNA when the dipodal silane and 8-arm PEG are used in combination, when compared to alternative conjugation strategies. Both 8mer and 22mer oligonucleotides are securely conjugated to the high-brightness nanoparticles and their availability to hybridize with a complementary strand is confirmed using solution-based DNA hybridization experiments. In addition, a full surface-based sandwich assay demonstrates the potential these nanoparticles have in the detection of less than 500 femtomolar of a DNA analogue of micro RNA, miR-451.


相关化合物

  • 硼氢化钠
  • 氯化钠
  • 溴化钾
  • 正己烷
  • 多库脂钠
  • 磷酸
  • 盐酸
  • 氨水
  • 磷酸氢二钠
  • L-赖氨酸盐酸盐

相关文献:

A new route for synthesis of silver:gold alloy nanoparticles loaded within phosphatidylcholine liposome structure as an effective antibacterial agent against Pseudomonas aeruginosa.

2015-03-01

[J. Liposome Res. 25(1) , 38-45, (2015)]

Development of Man-rGO for Targeted Eradication of Macrophage Ablation.

2015-09-08

[Mol. Pharm. 12 , 3226-36, (2015)]

Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles.

2015-06-10

[ACS Appl. Mater. Interfaces 7 , 12168-75, (2015)]

One Step Preparation of Reduced Graphene Oxide/Pd-Fe3 O4 @Polypyrrole Composites and Their Application in Catalysis.

2015-09-01

[Chem. Asian J. 10 , 1940-7, (2015)]

Surface modification-induced phase transformation of hexagonal close-packed gold square sheets.

2015-01-01

[Nat. Commun. 6 , 6571, (2015)]

更多文献...