Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition.
Changrong Zhao, Jie Wu, Huangxian Ju, Feng Yan
文献索引:Anal. Chim. Acta 847 , 37-43, (2014)
全文:HTML全文
摘要
An ultrasensitive multiplexed immunoassay method was developed by using streptavidin/nanogold/carbon nanohorn (SA/Au/CNH) as a novel signal tag to induce silver enhancement for signal amplification. The Au/CNH was prepared by in situ growth of nanogold on carboxylated CNH and functionalized with streptavidin. The SA/Au/CNH showed well dispersibility in physiological buffer and could sever as a common tracing tag to recognize biotinylated signal antibody. The immunosensor array was prepared on disposable screen-printed electrodes. Through sandwich-type immunoreaction and biotin-streptavidin affinity reaction, the SA/Au/CNH tag was captured on the immunoconjugates to induce silver deposition and amplify the electrochemical stripping signals. Using α-fetoprotein and carcinoembryonic antigen as model analytes, the proposed method showed wide linear ranges with the detection limits down to 0.024 pg mL(-1) and 0.032 pg mL(-1), respectively, and eliminated completely signal cross-talk between adjacent immunosensors. It provided a convenient, high-efficient and ultrasensitive electrochemical detection route for biological analytes, showing great potential in clinical application.Copyright © 2014 Elsevier B.V. All rights reserved.
相关化合物
相关文献:
2015-03-01
[J. Liposome Res. 25(1) , 38-45, (2015)]
2015-09-08
[Mol. Pharm. 12 , 3226-36, (2015)]
2015-06-10
[ACS Appl. Mater. Interfaces 7 , 12168-75, (2015)]
2015-09-01
[Chem. Asian J. 10 , 1940-7, (2015)]
2015-01-01
[Nat. Commun. 6 , 6571, (2015)]