Journal of Natural Products 2010-07-23

An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.

Yunbao Liu, Muraleedharan G Nair

文献索引：J. Nat. Prod. 73 , 1193-5, (2010)

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摘要

Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.

相关化合物

  • 槲皮素水合物
  • 硬脂酸
  • 1,2,3-苯三酚
  • 绿原酸
  • 抗坏血酸
  • 香草酸
  • 4-羟基苯甲酸
  • 叔丁基对苯二酚(TB...
  • 月桂酸
  • 正壬酸

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