Analytical and Bioanalytical Chemistry 2015-01-01

Identification of lipid- and protein-based binders in paintings by direct on-plate wet chemistry and matrix-assisted laser desorption ionization mass spectrometry.

Cosima Damiana Calvano, Inez Dorothé van der Werf, Francesco Palmisano, Luigia Sabbatini

文献索引：Anal. Bioanal. Chem 407(3) , 1015-22, (2015)

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摘要

Direct on-target plate processing of small (ca. 100 μg) fragments of paint samples for MALDI-MS identification of lipid- and protein-based binders is described. Fragments were fixed on a conventional stainless steel target plate by colloidal graphite followed by in situ fast tryptic digestion and matrix addition. The new protocol was first developed on paint replicas composed of chicken egg, collagen, and cow milk mixed with inorganic pigments and then successfully applied on historical paint samples taken from a fifteenth century Italian panel painting. The present work contributes a step forward in the simplification of binder identification in very small paint samples since no conventional solvent extraction is required, speeding up the whole sample preparation to 10 min and reducing lipid/protein loss.

相关化合物

  • 异丙醇
  • 甲醇
  • 碳酸氢铵
  • 三氟乙酸(TFA)
  • 苦鬼臼毒素
  • 甘油三月桂酸酯
  • 三硬脂酸甘油酯
  • 肉豆蔻酸甘油三酯

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