Journal of Nanobiotechnology 2015-01-01

Detection of residual rifampicin in urine via fluorescence quenching of gold nanoclusters on paper.

Krishnendu Chatterjee, Chiung Wen Kuo, Ann Chen, Peilin Chen

文献索引:J. Nanobiotechnology 13 , 46, (2015)

全文:HTML全文

摘要

Rifampicin or rifampin (R) is a common drug used to treat inactive meningitis, cholestatic pruritus and tuberculosis (TB), and it is generally prescribed for long-term administration under regulated dosages. Constant monitoring of rifampicin is important for controlling the side effects and preventing overdose caused by chronic medication. In this study, we present an easy to use, effective and less costly method for detecting residual rifampicin in urine samples using protein (bovine serum albumin, BSA)-stabilized gold nanoclusters (BSA-Au NCs) adsorbed on a paper substrate in which the concentration of rifampicin in urine can be detected via fluorescence quenching. The intensity of the colorimetric assay performed on the paper-based platforms can be easily captured using a digital camera and subsequently analyzed.The decreased fluorescence intensity of BSA-Au NCs in the presence of rifampicin allows for the sensitive detection of rifampicin in a range from 0.5 to 823 µg/mL. The detection limit for rifampicin was measured as 70 ng/mL. The BSA-Au NCs were immobilized on a wax-printed paper-based platform and used to conduct real-time monitoring of rifampicin in urine.We have developed a robust, cost-effective, and portable point-of-care medical diagnostic platform for the detection of rifampicin in urine based on the ability of rifampicin to quench the fluorescence of immobilized BSA-Au NCs on wax-printed papers. The paper-based assay can be further used for the detection of other specific analytes via surface modification of the BSA in BSA-Au NCs and offers a useful tool for monitoring other diseases.


相关化合物

  • 氢氧化钠
  • 利福平
  • 3-乙基-2,4-戊烷二...
  • 吡嗪酰胺
  • 盐酸乙胺丁醇

相关文献:

Aptamer-based polyvalent ligands for regulated cell attachment on the hydrogel surface.

2015-04-13

[Biomacromolecules 16(4) , 1382-9, (2015)]

Osmoregulatory bicarbonate secretion exploits H(+)-sensitive haemoglobins to autoregulate intestinal O2 delivery in euryhaline teleosts.

2014-10-01

[J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. 184(7) , 865-76, (2014)]

Polymerization of affinity ligands on a surface for enhanced ligand display and cell binding.

2014-12-08

[Biomacromolecules 15(12) , 4561-9, (2014)]

Continuous syntheses of Pd@Pt and Cu@Ag core-shell nanoparticles using microwave-assisted core particle formation coupled with galvanic metal displacement.

2014-08-07

[Nanoscale 6(15) , 8720-5, (2014)]

Effect of (2)H and (18)O water isotopes in kinesin-1 gliding assay.

2014-01-01

[PeerJ 2 , e284, (2014)]

更多文献...